Hon Bio Class 21

Today in class we went over the DNA replication process. Some of the important enzymes includes: 

We went step by step starting with the breaking of the hydrogen bonds by the Helicase enzyme: 

Then we moved on to the addition of RNA nucleotide which were put in place by RNA Primase. After that, DNA polymerase III added DNA nucleotides in the 3' - 5' direction. This left a lagging strand of DNA that lacked nucleotides.

RNA nucleotides are then added to the lagging strand but some spaces are still left open for the placement of DNA nucleotides. Polymerase I replaces RNA nucleotides with DNA nucleotides in the lagging strand to allow for phosphate diecer bonds to form. Ligase allows the okasaki fragments to bond and form a strand of new DNA.

We checked the results of our bacteria a found that only one of them glowed.